| abstract |
The invention relates to a-amylase variants that are stabilized by the point mutagenesis of asparagine (N-) radicals or glutamine (Q-) radicals, these radicals being located on the surface of the molecule, to form other amino acids with regard to a hydrolysis performed via the solvent, in particular, at elevated temperatures or high pH values. The invention also relates to methods for increasing the stability of an a-amylase with regard to a hydrolysis performed via the solvent, in particular, at elevated temperatures or high pH values, whereby at least one asparagine (N-) radical or glutamine (Q-) radical located on the surface of the molecule is exchanged to form another amino acid radical. The a-amylase variants obtained in this manner exhibit a better stability with regard to influences by solvents, an increased processivity and are suited for numerous technical areas of application, particularly for use as active ingredients in detergents and cleansers. |