Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_008949e88ec45543c04c2ac2405068a8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_864bfa4a0b74643642e9a550bbe5acc5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0bba768d26e9f58ca9c59d2d1bd66837 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1013 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1006 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2005-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_37bd562496d5b4cc54fba24d5762a2f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae0febac94faec35ea4a047571ffafe2 |
publicationDate |
2006-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2006015326-A2 |
titleOfInvention |
Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers |
abstract |
The present invention is directed to a method of isolating a target species (e.g., target nucleic acid species) from a mixture. In the methods of the present invention, the mixture is combined with solid phase carriers having a surface comprising multiple functional groups one of which reversibly and selectively binds the target species. In a particular embodiment, the mixture is combined with solid phase carriers having a first functional group which reversibly binds nucleic acids and a second functional group which selectively and reversibly binds the target nucleic acid species, thereby producing a first combination. The first combination is maintained under conditions appropriate for binding of the nucleic acids to the first functional group and binding of the target nucleic acid species to the second functional group. The solid phase carriers are separated from the first combination, and combined with an agent (e.g., buffer) that selectively removes (e.g., elutes) either the nucleic acid from the first functional group or the target nucleic acid species from the second functional group of the solid phase carriers, thereby isolating a target nucleic acid species from a mixture comprising a plurality of nucleic acid species. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7527929-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3294448-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114307988-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007035750-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-101532012-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021122855-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3002337-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008023208-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008114025-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3215620-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9610584-B2 |
priorityDate |
2004-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |