abstract |
Disclosed are an analysis kit and a measuring method of the copy number of the chromosome, gene or specific nucleotide sequence. According to the invention, one or more artificial single nucleotide polymorphism (SNP) sequences including one or more artificial SNP having a base change introduced artificially are used, the artificial SNP sequences, including a primer sequence part, are same as a wild-type genomic DNA sequences, except the artificial SNP. According to the invention, it is possible to obtain more accurate values than the other molecular methods determining the copy number of the specific gene or nucleotide sequence as well as to remarkably reduce the measurement time and the required manpower. |