| abstract |
The invention relates to a peptide substrate selectively recognisable by a botulinum toxin A, BoNT/A comprising a Nop-(Z)-Pya fragment in the peptide structure thereof, wherein Z is an aminoacid chain, preferably RA and said fragment is cleaved by the toxin. Said invention also relates to the substrate use, in particular for carrying out methods for detecting, identifying and/or dosing the botulinum toxin A at very low concentrations (of order of 20 pg) or the inhibitors and/or activators of the toxin metallopeptidase activity, thereby making it possible to quantify the power thereof. |