| abstract |
The present invention relates to a lateral flow assay and system, including a test strip, for detection and quantification of analytes in samples, where the assay is volume independent, and the sample size is less than about 100 µl, where the test strip includes a first membrane (12, 18) that is in capillary contact with an optional second membrane, the second membrane (9), if present is in capillary contact with an optional third membrane (13), containing a mobilizable detectable agent, or with a fourth membrane (9), all such membranes being in fluid contact with a fifth membrane (14), a sixth membrane (15), optionally a seventh membrane (16), a capture band (5) for capturing the analyte and at least one control band (6, 7), where the test strip is configured to support removal of red blood cells from the sample and to allow uni-directional or bi-directional fluid flow of fluid. |