http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005116208-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e1c9e4da6a6d96fb4fa8ebc80731631 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e726f44d81f927d0c1cdb6540437181d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_65187685f200cc85a47c5be2154489b8 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2005-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f084bbbec2098e47cc4b9687b0d6d0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc88dd3f73ad30725ae30016b99da07e |
| publicationDate | 2005-12-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2005116208-A1 |
| titleOfInvention | Dna amplification method |
| abstract | It is intended to provide a DNA amplification method whereby a single-stranded DNA in a desired direction and in a desired region can be conveniently and efficiently prepared. Namely, a DNA amplification method with the use of a DNA as a template which comprises: the first step wherein use is made of a first primer being complementary to the 3’-terminal side of a target base sequence, a second primer having a base sequence being homologous with the 5’-terminal side of the target base sequence, and a third primer having a base sequence in the 3’-terminal side being homologous with the second primer or a part of the 5’-terminal side base sequence of the first primer, the melting temperature of the first primer Tm1, the melting temperature of the second primer Tm2 and the melting temperature of the third primer Tm3 have the relationships represented by the following formulae: (formulae) Tm1<Tm3 and Tm2<Tm3 and a PCR cycle is carried out at an annealing temperature Ta (provided that Ta≤Tm1 and Ta≤Tm2); and the second step wherein a PCR cycle is carried out at an annealing temperature Tb (provided that Tm1<Tb, Tm2<Tb and Tb≤Tm3). |
| priorityDate | 2004-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.