http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005108586-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_04e04aaf2e6e7215e508fdaaeb5af115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec4cc38d6f7b75a5b30793a992fd4194 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
filingDate | 2005-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0a35146e35b8307c5337e942fa6c38a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63f4288c69ab78232fecc295f9def625 |
publicationDate | 2005-11-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2005108586-A1 |
titleOfInvention | A method for increasing the ratio of homologous to non-homologous recombination |
abstract | Gene targeting allows the deletion (knock out), the repair (rescuing) and the modification (gene mutation) of a selected gene and the functional analysis of any gene of interest. Targeting of nuclear genes has been a very inefficient process in most eukaryotes including plants and animals due to the dominance of illegitimate integration of the applied DNA into non-homologous regions of the genome. The present invention provides a method for increasing the ratio of homologous to nonhomologous recombination of a polynucleotide into a host cell's DNA by suppressing non-homologous recombination. Surprisingly, the number of non-homologous recombination events can be reduced if the polynucleotide is applied as a purified singlestranded DNA, preferably coated with a single strand binding protein. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10584363-B2 |
priorityDate | 2004-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.