| abstract |
An Indian isolate of Hepatitis A virus- NIVIN97 has been isolated, adapted to tissue culture, characterized and further propagated using Vero and MRC-5 cell lines for vaccine preparation. The method involves the cell culture adaptation of the virus isolate from clinical sample (faecal) in BGMK cell line initially , characterization of the virus and further adaptation to Vero and MRC-5 cells , scale-up ,inactivation and down stream processing method of the inactivated viral antigens for the preparation of an inactivated vaccine. |