| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_057ceccf9a991327cd61ca7b14239636
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ffc50cc1a8b55071f575b2020093c21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_841973573ab3c94756016756da81957c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9edaceabb7e40017a01363f056ad6ef6 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00653
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00596
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00585
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00527
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00441
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00432
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00497
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00729
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00725
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00677 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5438
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2004-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3c8f8021815f5d73440ca23b361256c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_06025b2d3fc2d294aa97b9eb7ac80b57
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61ceee82e67c3286cd91813f879095fe |
| publicationDate |
2005-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2005059181-A1 |
| titleOfInvention |
Carbon electrode surface for attachment of dna and protein molecules |
| abstract |
Methods for conducting an assay to detect nucleic acid hybridization are disclosed. In particular, the fabrication of a carbon electrode suited to nucleic acid hybridization detection is described. In some preferred embodiments, a micro array of carbon electrodes is constructed using photolithography. The final step in the photolithography process involves developing a solder mask 306 with an alkaline solution. The alkaline solution oxidizes the carbon surface 304 producing surface carboxylic acid groups. The surface carboxylic acid groups are reacted with EDC or DCC and NHS to produce NHS esters. Immobilization of NeutrAvidinTM onto the electrode surface is effected by reaction with the surface NHS ester groups. A biotinylated probe DNA molecule is then attached to the electrode via binding between the biotin group and the immobilized NeutrAvidinTM. The resulting electrode can be used to detect hybridization between the probe DNA and complementary target DNA. |
| priorityDate |
2003-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |