Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8c98c9ae6518d517660f0f91ca7fcd73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7415302f46f7fc4d53ae6bb2b5f7ce83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4798eb5470fe565466800b95ae4a5805 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0eb9b0fce0c776512a26b2bab4a9a268 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e3450ad2d0b06dec87233cc9b194042a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4f624623087fa9d6b9c35b69391c9b69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_341c78007c3be86349b1b7e7cfa4da97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c16e1c811b667aa497d09459e6a3ee5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5ad09028b761c40ad03333afdea62320 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-565 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-06139 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-565 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-078 |
filingDate |
2004-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b146638c32514231f6c0f5d279bd23b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f319ee817fc4e8c91b78cee711c73965 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cbb3ed7abca7fd91c3677b5d7faffcb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_476b9fc24bb07dd7eb35aead7dc02c70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d97a6f8bf9e2e4578d1100c757b5cd1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a5d3cc7b3f0086fc63b1a15771a0aa31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3738dc19bd207cf532a5081ec35164a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99bc07894a254ce240ac62003709fd9d |
publicationDate |
2005-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2005054288-A1 |
titleOfInvention |
Process for purifying interferon beta |
abstract |
Provided is a process for purifying human interferon beta from a recombinant human interferon beta-containing culture comprising performing affinity chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), wherein the affinity chromatography includes: adsorbing the interferon beta-containing culture to an equilibrated affinity chromatography column, followed by washing with an equilibration buffer solution; washing the column with a washing buffer solution A of pH 6.5-7.5 containing 30-60 wt% of propylene glycol and a washing buffer solution B of pH 6.5-7.5 containing 10-30 wt% of propylene glycol and 1-2M NaCl; and eluting a human interferon beta-containing fraction with a buffer solution of pH 6.5-7.5 containing 40-60 wt% of propylene glycol and 1-2M NaCl. |
priorityDate |
2003-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |