| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22e3f121642d219dcf6a8f148ea9e2b5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad321af1f1109bed07145e79497ea036
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44e1d9ff2fb73293200a1e0cbb0395e8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc29ef8e5b1a85c5a365d14b4b98232f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e49daa5a709f62dc8a7fdc98a7c3599
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_abba33b1872508b2db951bf2e482cd07 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate |
2004-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50eafe98b73f8d369674a07d817bd8c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5f764dfe7e853d542a4db1f61931608
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f094626c52ad2e59a16208d54565c5b7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0767f7f42e3c0c770cbb54b02dbee20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6646001f3408ef9ae5cba89944e92025 |
| publicationDate |
2006-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2005038460-A3 |
| titleOfInvention |
Site-specific labeling of affinity tags in fusion proteins |
| abstract |
The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a polyhistidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. |
| priorityDate |
2003-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |