Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_abba33b1872508b2db951bf2e482cd07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad321af1f1109bed07145e79497ea036 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22e3f121642d219dcf6a8f148ea9e2b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e49daa5a709f62dc8a7fdc98a7c3599 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44e1d9ff2fb73293200a1e0cbb0395e8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc29ef8e5b1a85c5a365d14b4b98232f |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-60 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-13 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-13 |
filingDate |
2004-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50eafe98b73f8d369674a07d817bd8c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e5f764dfe7e853d542a4db1f61931608 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f094626c52ad2e59a16208d54565c5b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6646001f3408ef9ae5cba89944e92025 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0767f7f42e3c0c770cbb54b02dbee20 |
publicationDate |
2005-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2005038460-A2 |
titleOfInvention |
Site-specific labeling of affinity tags in fusion proteins |
abstract |
The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a polyhistidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9535070-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2014141991-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113943372-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007231124-A |
priorityDate |
2003-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |