Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dc9759709c7173fdc7634c6583aeadfa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1763f7f78d01cad638210d060676591a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df52481b28c8ce51d9f1b8052f994ed3 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate |
2004-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8c4946b8c43651a17aa984922acc0ad6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e875f21c90dd6607f9897f11f2800e88 |
publicationDate |
2005-04-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-2005030995-A1 |
titleOfInvention |
Methods of synthesizing polynucleotides using thermostable enzymes |
abstract |
Disclosed are methods of synthesizing a polynucleotide complementary to a target polynucleotide include steps of subjecting a nonĀ-thermophilic cell comprising a thermostable polymerase to a temperature effective to disrupt the cell to form a reaction mixture, wherein the reaction mixture comprises the target polynucleotide and one or more primers that hybridize to a sequence of the target polynucleotide or to a sequence flanking the polynucleotide and incubating the reaction mixture under conditions whereby the polynucleotide is synthesized. Also disclosed are cell libraries and kits in accordance with the invention. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10858385-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10421953-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10316342-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20190003553-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10704067-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10036001-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10577635-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11274284-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-102536687-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2017246458-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017176963-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-109196109-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10954541-B2 |
priorityDate |
2003-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |