| abstract |
A process for producing ribonucleotide or a ribonucleotide derivative from a ribonucleotide derivative having a 2'-hydroxy group protected by a protective group which can be efficiently introduced into the 2'-hydroxy group and causes no steric hindrance in condensation reaction, and which is stably present under the conditions to be used for the removal of a protective group for the 5'-hydroxy group, removal of a protective group for the phosphoric diester group, removal of a protective group for a nuclei acid base, separation of the nucleotide chain from a solid-phase support, etc. but can be removed in a short time under conditions different from these conditions. The process comprises fluorinating a ribonucleotide derivative (II) represented by the following formula (II): (II) or comprises treating the derivative with a tertiary amine in a water-free solvent in the presence of a silylating agent and then hydrolyzing the derivative under neutral or acidic conditions. Thus, a nucleotide or nucleotide derivative from which the 2-hydroxy-protecting group has been removed is produced. |