| abstract |
The invention relates to a method for creating a cyclic form of peptides and proteins, in which method linear thioesters act as substrates. The cyclic formation is catalysed by thioesterase domains of NRPS or PKS cyclases. The inventive substrates consist of a linear peptide, to which a charge-stabilised aromatic, heteroaromatic or araliphatic leaving group is bonded. Said substrates permit high yields and reaction speeds of linear peptides that were created in cyclic form in prior art to be obtained and also permit the creation of a cyclic form for those peptides, for which such a process was previously impossible. |