| abstract |
A reaction container for carrying out the operations of nucleic acid extraction and nucleic acid amplification, which are different from each other in operation process, in a single container. In particular, a reaction container for detecting a target nucleic acid from a sample, comprising at least a first chamber in which an extraction reagent composition for extracting the nucleic acid from the sample is placed, a second chamber in which an amplification reagent composition for amplifying the target nucleic acid is placed, means for separating the first chamber and the second chamber from each other and an opening realizing introduction of the sample into only the first chamber. The above separation means disengages the separation of the first chamber from the second chamber by physical energy from outside the reaction container, thereby realizing mixing of the extraction reagent composition of the first chamber with the amplification reagent composition of the second chamber. |