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publicationDate 2005-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-2005001736-A2
titleOfInvention Intracellular metabolic flux analysis method using substrate labeled with isotope
abstract A method for analyzing an intracellular metabolic flux comprising determining the intracellular metabolic flux from analytical values of cells cultured in a medium containing an isotope-labeled substrate as a carbon source on the basis of an intracellular metabolic flux model constructed for the intracellular metabolic flux to be analyzed, wherein (a) influence of an exchange reaction between an intracellular metabolite and a cell component produced by integration of the intracellular metabolite is considered, (b) uptake of a compound in a medium into cells, which compound is identical to an intracellular metabolite and unlabeled with an isotope, is considered, or (c) carbon dioxide produced in a production reaction.
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