| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_38c24fde5f99c1cdc06bf70936bd4a9d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6b2787fb8ac35a9508a61655fbcf73db |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2004-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3cabe7879104ad186d9ef00c9b761bc1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9ed3395445d072e64b0cd258d8a7ab6 |
| publicationDate |
2004-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2004113567-A2 |
| titleOfInvention |
Improved heavymethyl assay for the methylation analysis of the gstpi gene |
| abstract |
Described herein is a method for the detection of cyto-sine methylation in DNA samples, wherein the following steps are conducted: a genomic DNA sample, which comprises the DNA to be investigated as well as background DNA is treated with bisulfite (= disulfite, hydrogen sulfite) in such a way that all of the unmethylated cytosine bases are converted to uracil, while the 5-methylcytosine bases remain un-changed; the bisulfite treated DNA sample is amplified with the use of at least 2 primer oligonucleotides as well as a polymerase, wherein the DNA to be investigated is pre-ferred over the background DNA as the template, and a control fragmentis amplified simultaneously to the am-plification of the bisulfite treated DNA within the same reaction mixture the amplified products are analyzed and the methylation status in the DNA to be investigated is concluded from the presence and /or the amount of the amplified products and/or from the analysis of additional positions. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-201908624-D0
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1071815-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1071815-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020254405-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1764419-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020254364-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005093095-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2021130356-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007075114-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1764419-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020165361-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2019122303-A1 |
| priorityDate |
2003-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |