| abstract |
The invention provides an apparatus for maintaining a closed continuous anhydrous system for automated polynucleotide synthesis, having a) a plurality of moisture resistant reagent containers, b) a dry box capable of forming a seal over a synthesis platform of an automated polynucleotide synthesizer, c) moisture-resistant tubing connecting the reagent containers to the dry box, d) a reagent gas feed connecting the reagent containers to a gas, where the gas pressurizes the reagent containers, and e) a digital gas regulator connected to the reagent gas feed, where the gas regulator maintains constant pressure in the reagent containers. |