http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004083461-A9
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5de9f3bf3c8d6d271d5c99095c016462 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_188dbfaff3cfc9b076c138a05613432d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01b130399797df356efc896783699a2d |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1041 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate | 2004-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_51edf11e6cf5098fd5e01c6fa212b2c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15ec52f60524b8acf91a97e9e1c294db |
publicationDate | 2004-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-2004083461-A9 |
titleOfInvention | Recovery of dna |
abstract | The invention provides a method for recovery of cDNA from mRNA, comprising reverse transcription (RT) of mRNA using a RT primer which includes a sequence based on the 5' consensus region of the mRNA which is identical or similar to 5' consensus region of the mRNA and which includes a sequence capable of specifically hybridising to the 3' region of the mRNA, followed by polymerase chain reaction (PCR) using a single primer to generate ss cDNA, ds cDNA and amplify the cDNA. Primers for use in methods of the invention and kits for performing methods of the invention are also provided. The methods of the invention can partially or fully automated. |
priorityDate | 2003-03-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 122.