http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004072252-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4589b30807b53be5b8d5c09bfad19dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a66c5e8d7bea9a2536851d97833078cb |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- |
| filingDate | 2004-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8c2e42c7fc9336c8e53bf9d20dd8222 |
| publicationDate | 2004-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2004072252-A2 |
| titleOfInvention | Polymerase-based protocols for the introduction of deletions and insertions |
| abstract | The present invention relates to improved methods of introducing site-directed mutations into circular DNA without the need for subcloning. The invention comprises single-stage, polymerase-based, mutagenesis procedures which eliminate the need for the second-stage linear cyclic amplification reaction of the prior art. In accordance with the invention, production of complimentary mutagenized DNA strands is accomplished in separate, single-primer, linear amplification reactions carried out for each primer of a mutagenic primer pair. The mutagenized DNA strands produced in the separate primer reactions are combined and annealed and the resulting double-stranded mutagenized DNA intermediate may then be used directly to transform a host cell for further production of the desired mutant DNA. Major applications of this method include directed evolution and other areas that benefit from the development of diversity. |
| priorityDate | 2003-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 165.