http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004072245-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a66c5e8d7bea9a2536851d97833078cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4589b30807b53be5b8d5c09bfad19dc |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N- |
| filingDate | 2004-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e8c2e42c7fc9336c8e53bf9d20dd8222 |
| publicationDate | 2004-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2004072245-A2 |
| titleOfInvention | Polymerase-based protocols for the introductions of deletions and insertions |
| abstract | The invention relates to primers, libraries of primer, kits and methods for site-specific in vitro mutagenesis comprising: (a) cloning a parental polynucleotide into a vector comprising a cloning site, thereby obtaining a cloned product; (b) denaturing the cloned product, thereby obtaining a single-stranded polynucleotide template; (c) hybridizing at least one mutagenized oligonucleotide primer to the single-stranded polynucleotide template, thereby obtaining a first heteroduplex; (d) subjecting the first heteroduplex to linear amplification, thereby obtaining amplified products; (e) reacting the amplified products with a ligase, thereby obtaining ligated products; (f) denaturing the ligated products, thereby obtaining single stranded mutated polynucleotides; (g) hybridizing the single stranded mutated polynucleotides with a second oligonucleotide primer thereby obtaining second hybridized complexes; (h) copying the second hybridized complex and ligating the double stranded product thereof; thereby obtaining a circular double stranded mutated polynucleotide; (i) transforming the double-stranded mutated polynucleotide into a bacterial host, thereby obtaining transformants. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006050062-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006050062-A3 |
| priorityDate | 2003-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.