http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004063373-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_532aad28635832abf63bb7d0102d25ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_14430ce0c28e36044927f5834e4d02ad |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d8e27b8f12a078a7b8e96fcec2fa84a1 |
| publicationDate | 2004-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2004063373-A1 |
| titleOfInvention | Dna size markers and method for preparing them |
| abstract | The present invention relates to a method for preparing DNA size markers by virtue of multiplex polymerase chain reaction and DNA size markers prepared thereby. The present method comprises the steps of: (a) selecting a plurality of specific regions on target DNA molecule(s) to be amplified so that each amplified product has a definite and desired length; (b) preparing a plurality of first primer pairs for amplifying the specific regions in which a 5'-end portion of each forward primer of the first primer pairs has a substantially common nucleotide sequence, a 5'-end portion of each reverse primer of the first primer pairs has a substantially common nucleotide sequence and a 3'-end portion of the first primer pairs has an annealing nucleotide sequence substantially complementary to a site on the specific region to anneal thereto; (c) performing polymerase chain reaction of the specific regions using the first primer pairs so that DNA molecules with definite and desired lengths are amplified; and (d) performing multiplex polymerase chain reaction of the amplified products in the same reaction using one type of a third primer pair in which each third primer comprises a nucleotide sequence to be hybridized with a nucleotide sequence at each end portion of the amplified products. |
| priorityDate | 2003-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.