http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004048580-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec7aa9366c4867ba95ac5cd4fbbaec25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_df62e086c8a027fa1ebeab2c3fb261cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76a1487705d0cb388375a2738f3a8887 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8209 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2003-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_140c0a130278392c2456b4aecc83f8f0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56aa23a07202e0e79099a6a3ef308789 |
| publicationDate | 2004-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2004048580-A1 |
| titleOfInvention | A method for marker-less integration of a sequence of interest into the genome of a cell |
| abstract | The present invention provides multiple methods that result in a site-specific, marker-less integration of a sequence of interest. The methods are based on the following principles: - The genomic presence in a cell (host cell) of a selectable or screenable gene X. As a result of a mutation, this gene X can be essentially sensitive or insensitive to a certain component or condition Z, or as a result of a mutation in said gene X, the host cell is made dependent on the presence of a certain component or condition Z; - A plasmid on which the desired insertion (or deletion) is present, further harbouring a truncated gene X and a selectable marker (such as an antibiotic resistance gene) to select or screen for the presence or absence of vector sequences in the host cell; - Positive selection of the final recombination step, avoiding complicated and time-consuming screening for the desired recombinants. In a preferred situation, both recombination steps can be positively selected. - When the starting host cell contains a mutant gene X on the genome, the final recombinant has a gene X without a mutation and the genome further only comprises the desired insertion or deletion. When the starting host cell contains an original gene X, the final recombinant bears a mutation in gene X and the genome further only comprises the desired insertion or deletion. |
| priorityDate | 2002-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.