http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004042034-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0aa3d28e6cc58fca713a6a24a687781e http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e0daec85fdd4a4c04e22225e70a5cd9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_55b6ebede0bf67a71ab4f8ea9b2525c2 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6865 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd9fe921ab108cd324363707f2e359f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c18e9887e77886318d488b0f6a7479e5 |
| publicationDate | 2004-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2004042034-A2 |
| titleOfInvention | Method for the linear amplification of complementary dna |
| abstract | In one aspect, the present invention provides methods for linearly amplifying cDNA from an RNA molecule. The methods comprise the steps of: (a) synthesizing an antisense cDNA molecule using an RNA molecule as a template; (b) synthesizing an extended antisense cDNA molecule using a template-switching oligonucleotide as a template; (c) synthesizing multiple copies of sense cDNA molecules using the extended antisense cDNA strand as a template; and (d) generating double-stranded cDNA molecules using the multiple copies of sense cDNA molecules as templates. Another aspect of the invention provides methods for linearly amplifying RNA, comprising the steps o£ (a) using an RNA molecule as a template for synthesizing an antisense cDNA molecule; (b) using a template-switching oligonucleotide as a template for synthesizing an extended antisense cDNA molecule; (c) using the extended antisense cDNA molecule as a template for synthesizing multiple copies of sense cDNA molecules; (d) using the sense cDNA molecules as templates for generating double-stranded cDNA molecules; and (e) using the double-stranded cDNA molecules as templates for synthesizing RNA molecules. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2007030505-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9808532-B2 |
| priorityDate | 2002-11-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 277.