| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cf2a450f51353cd86d04cde97846fac2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_43c8ac3c49dd913a1db4da7943973a14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_277e2f81c76a83eff7d6f87b886b01f3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4e0879f09f869d6109111ef67f1631cb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bbadc41aa91592752369d88100f96411
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d665ab1c713018ff2c6845faa494f678 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 |
| filingDate |
2003-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3396b5e37429a5554a5e83e9b29ef129
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90b187da3f3bd09bc8fc27724312518a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6af77dd8232fb62af534090a6572e79e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b972060f59c8917514690d604d4ea8d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a0a7d122e1682f5ea9acc062e43d1bca |
| publicationDate |
2004-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2004029209-A2 |
| titleOfInvention |
Epitope-tagged recombinant growth arrest specific gene 6 protein |
| abstract |
The present invention provides epitope-tagged mammalian recombinant Growth Arrest Specific Gene 6 (Gas6) proteins or polypeptides that retain their biological activity of Gas6, including a novel method of generating and purifying the aforesaid proteins or polypeptides. Such biolgically active epitope-tagged Gas6 proteins or polypeptides can be used when the presence of Gas6 is required and/or when Gas6 antagonists and receptors need to be identified. Prior to being expressed from a mammalian cell, the recombinant Gas6 protein or polypeptide is tagged with either Flag or polyhistidine to the C-terminus. After expression, the protein or polypeptide is purified in a single step affinity purification using either anti-Flag antibody resin or nickle resin. The purified epitope-tagged mammalian recombinant Gas6 protein or polypeptide maintains its biological activity and can be used thereafter. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004092735-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004092735-A3 |
| priorityDate |
2002-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |