| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f4976a3e45d71cbea2f1e51cd08f67ee
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a00ab7d3979dc8b100017a99bea8a1ff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d53e55c12ffa42dea854d90ecccb39b9
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9fd5db57980fbec997a801270d54dd49 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-50 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-07
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-08 |
| filingDate |
2003-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ca6e1b58331b7a289abeeb4cdd16565
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3df156e744740a9bb1b467ba92f0399
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c88d0a591f28b2661327725b754b8ae9 |
| publicationDate |
2004-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2004027043-A2 |
| titleOfInvention |
Method for production of a multimeric protein by cell fusion |
| abstract |
The present invention features methods of producing a multimeric protein in a hybrid, recombinant cell, as well as for identifying recombinant cells expressing heavy chain which cells are useful in production of such hybrid cells. In the method of the invention, at least first and second recombinant cells are engineered to contain a first and second expression cassette, respectively, which expression cassettes contain an amplifiable marker and encode first and second components of the multimeric protein. The recombinant cells are then fused to produce a hybrid cell expressing the multimeric protein. The hybrid cell is then cultured to provide for amplification of the DNA encoding the first and second multimeric protein components. The methods of the invention are widely applicable to the production of proteins having two or more components. |
| priorityDate |
2002-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |