http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004016806-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_11b9630a5cd1890097cf52ba0d99ce99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7b1ad3a371748600a06b43090cd343ef |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbcb5c7c94fd018a3db5082840e7c2aa |
| publicationDate | 2004-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2004016806-A1 |
| titleOfInvention | Using a polymerase with 3' to 5' exonuclease activity to perform gene sequence analysis |
| abstract | The present invention discloses a method to carry out gene sequence analysis using a polymerase with 3' to 5' exonuclease activity, which comprises the following steps: a. Prepare specific primers set with their 3' terminal modified; b. Subject the polymerase with 3' to 5' exonuclease activity to form a molecular 'on/off' switch with the modified primers, and perform selective primer extension reactions with the involvement of the said molecular 'on/off' switch; c. Detect image of the primer extension products and analysis the sequences. With the molecular switch of the present method, the accuracy is higher than the conventionally employed primer extension reactions mediated by a polymerase without proofreading function; It can also be used for direct analysis of unknown sequences, and it needn't to rely on a segment of know sequence when sequencing with the present method; Every signal obtained in this method gives information for one of more base sequences; The present method provides a high throughput analysis approach for Single Nucleotide Polyphism assay and gene sequence analysis. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2006141255-A |
| priorityDate | 2002-08-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.