| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_95c1b7115b19a6ae82cf6988529a5490
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bc10898deaadd5a1818f4e222280d872
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e2637725839ab38b24679c05b11407e2 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8206
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M35-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-42 |
| filingDate |
2003-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74a9f4d45a5c81fadf44ee6b4137abbd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adb1445b3e101f30e765b77c4199bf32 |
| publicationDate |
2004-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2004007736-A1 |
| titleOfInvention |
Electroporation method including the use of depressurization/pressurization |
| abstract |
An object of the present invention is to establish a transformation method simpler and more rapid than conventional techniques. This object can be solved by a method comprising the steps of a) holding a cell under a pressure different from an atmospheric pressure, and b) placing the cell and a nucleic acid under conditions capable of inducing electroporation. The present invention removes the necessity of culture which is conventionally required after gene transfer. Therefore, the present invention makes it possible to obtain a transformant of species which are impossible or considerably difficult to transform by conventional techniques. The simple transformation method of the present invention easily allows large-scale processing and large-scale analysis for research and development in the art. In addition, the present invention triggers dramatic advances in research, potentially leading to the development of innovative recombinants. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2006038571-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11713465-B2 |
| priorityDate |
2002-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |