| abstract |
A novel ferrocene compound; a reagent containing the compound; and a high-sensitivity method of determining a vitamine D compound with the reagent. Specifically, a ferrocene compound represented by the following formula (1): (1) is reacted with a VD compound, and the compound comprising these compounds combined with each other is subjected to LC/ESI-MS/MS. Thus, the VD compound can be determined with higher sensitivity than in conventional techniques. The ferrocene compound is extremely useful as an agent for derivative formation when a VD compound is determined by LC/ESI-MS/MS. The compound obtained, which comprises the ferrocene compound and VD compound which have been combined with each other, is useful as, e.g., a labeled compound in the determination of a VD compound by LC/ESI-MS/MS. |