| abstract |
GDP-mannose-3',5'-epimerases from algae have been identified, isolated, purified, and/or cloned. Disclosed herein are algal GDP-mannose-3',5'-epimerases from any algae, including those of the genera Chlamydomonas, Prototheca, and Chlorella. Also disclosed herein are nucleic acid and amino acid sequences for algal GDP-mannose-3',5'-epimerases and the use thereof to produce genetically modified host cells, as well as in methods to produce L-galactose, ascorbic acid, or intermediate products in the ascorbic acid biosynthetic pathway. |