| abstract |
The present invention relates to a method for the production of a cellular allogeneic vaccine,nwhich is based upon an allogeneic APC, comprising the following steps: a) isolationnof an APC from a subject or providing an APC already established and/or isolatednfrom a myeloid leukemia cell line, and b) modifying the APC with an antigen usingnany of the following methods : pulsing, transfection, infection or fusion; c)ntreatment using an agent capable of removing sialic acid on cell surfaces; andnoptionally d) culturing the APC in a suitable medium, an allogeneic vaccine obtainablenby the above method, a composition comprising said vaccine and a pharmaceutically acceptablencarrier and therapeutic use of said vaccine or said composition. |