http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03102232-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a8420eab41110e8ee0972916db8cc822 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b4036ff39a98c628548566e583cd0233 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db5c96f74bd5f3f369672c3a3f8be253 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2003-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5b14e3de39f99ecbe3e3d6aaff63daf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b7411ce4d106c53464c9ec756f551ee |
| publicationDate | 2004-02-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-03102232-A3 |
| titleOfInvention | Amplification of ribonucleic acids |
| abstract | The invention relates to methods for amplifying ribonucleic acids, comprising the following steps: (a) a DNA single strand is produced from an RNA by reverse transcription with the aid of a single-stranded primer having a defined sequence, an RNA-dependent DNA polymerase, and desoxyribonucleoside triphosphates; (b) the RNA is removed; (c) a DNA duplex is produced by means of a single-stranded primer comprising a box sequence, a DNA polymerase, and desoxyribonucleoside triphosphates; (d) the duplex is separated into single strands; (e) DNA duplexes are produced from one of the single strands obtained in step (d) by means of a single-stranded primer which comprises the sequence of a promoter in the 5' range and the same defined sequence as the primer used in step (a) in the 3' range, a DNA polymerase, and desoxyribonucleoside triphosphates; (f) a plurality of RNA single strands, both ends of which comprise defined sequences, are produced by means of an RNA polymerase and ribonucleoside triphosphates. The invention also relates to kits for amplifying ribonucleic acids according to one of said methods, said kits comprising the following components: at least one single-stranded primer comprising the sequence of a promoter; (b) at least one single-stranded primer comprising a box sequence; (c) an RNA-dependent DNA polymerase; (d) desoxyribonucleoside triphosphates; (e) a DNA-dependent DNA polymerase; (f) an RNA polymerase; and (g) ribonucleoside triphosphates. |
| priorityDate | 2002-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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