http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03100070-A2
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_183199e64ceb952c8396756995c2f77c http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a17fcb5c2bc74fb9cb9ba98bb8a96ff9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dbbac8bfb03e73154d01d3ccd269e281 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9911f3ffb183144488431472d9039dfa http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3ec960d6bbfaa93d9ffbed751f45824f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6014d32744b41d9c7927993182e3b50e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2840-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-60 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2003-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9de88afdf0815cf7b1a4aa863035727e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5ba9ed24be5d414a7b6144ef3242145b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_914a5b2f82d0f734764019dc33e8b342 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a78b8f0098fc74ab749499143c2f325 |
| publicationDate | 2003-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-03100070-A2 |
| titleOfInvention | The frog prince, a transposon vector for gene transfer in vertebrates |
| abstract | The present invention relates to a transposon-based DNA integration system comprising (a) a transposon which is devoid of a polynucleotide encoding a functional transposase and which comprises a polynucleotide of interest, wherein the transposon comprises inverted repeats having a degree of identity with the repeats within SEQ ID NO: 2 and its inverted repeat, respectively, of at least 90%; and (b) a transposase having at its N-terminus a DNA binding domain comprising the sequences of SEQ ID NO: 3 and 4; or (c) a polynucleotide encoding the transposase of (b). The present invention further relates to a method of transferring a polynucleotide of interest into cells of a vertebrate comprising the step of introducing the transposon-based DNA integration system of the invention into said cells. In addition, the invention relates to a method of effecting RNAi comprising (a) stably introducing a transposon comprising an expression cassette expressing a short interfering RNA and a selectable marker gene as part of the trans poson-based DNA integration system of the invention into a cell; (b) selecting for cells expressing the selectable marker; and (c) assessing whether the transcription/translation of the desired gene is effected by RNAi. A further embodiment of the invention is a method of gene trapping genes comprising the steps of (a) introducing the transposon-based DNA integration system of the invention into a cell; and (b) assessing for the expression of a selectable marker wherein expression of a selectable marker is indicative of integration of the transposon into a transcibed gene of the cell. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8765470-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8598328-B2 |
| priorityDate | 2002-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 111.