| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e83d6edbb2df5f8b382c7b36bb08966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e33153c8c16ca622172f764e6571db21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a4ea1a6fe171cd25b88767fe1dfe74c |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-43595 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-435
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-542
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 |
| filingDate |
2002-12-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc672a2164946f30177c689c43f63ac0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b3e7f4e2b47ba4ef41c96c7a0095452f |
| publicationDate |
2003-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-03089464-A1 |
| titleOfInvention |
Two green fluorescent protein fragments and their use in a method for detecting protein - protein interactions |
| abstract |
Fluorescence complementation products with intensity levels mimicking the full length intensities are obtained by introduction of improved folding capabilities with a mutation in position preceding the chromophore. This is particularly seen with the yellow variant of Green Fluorescent Protein (GFP). An additive increase is obtained by splitting the GFP between amino acids 172 and 173. Screening for drugs capable of preventing interaction between proteins is performed by selecting the cells with the highest dynamic range through Fluorescence Activated Cell Sorting (FACS), as illustrated with the ability of FK506 to break the rapamycin induced interaction between FRB and FKBP. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1616186-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2007514933-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1616186-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005052588-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005052588-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2317318-A1 |
| priorityDate |
2002-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |