| abstract |
A method for the detection of mutations in nucleic acids is disclosed, comprising a) a number of nucleic acid strands for analysis are brought into contact with at least two types of oligonucleotides, receptor oligos and probe oligos, under conditions which bring about hybridisation of the oligos with the DNA for analysis to give a sample complex and a reference complex in such a manner that a double-sided linkage of sample complex and reference complex is produced which is or may be immobilised, b) the linkage is then optionally immobilised, c) a tensile force is applied to the linkage until one of the complexes separates and d) whichever of the complexes has separated is determined. |