| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff3e741d8c3082a79ba472a5a4c2bef8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8d6a0a6e6962b2fef81a7bccaaec5c9d |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2003-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a28d31c25ca10ae9dd2e0e9fb37203b9 |
| publicationDate |
2003-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-03066895-A2 |
| titleOfInvention |
Quantitative methylation detection in dna samples |
| abstract |
Described is a method for methylation detection in a DNA sample. An isolated genomic DNA sample is treated in a manner capable of distinguishing methylated from unmethy-lated cytosine bases. The pretreated DNA is amplified us-ing at least one oligonucleotide primer, a polymerase and a set of nucleotides of which at least one is labeled with a first type of label. A sequence-specific oligonu-cleotide probe, marked with a second type of label, hy-bridizes to the amplification product and a FRET reaction occurs if a labeled oligonucleotide is present in close proximity in the amplification product. The method deter-mines the level of methylation of a sample by measuring the extent of fluorescence resonance energy transfer (FRET) between the donor and acceptor fluorophore. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8163485-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8273528-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8361719-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7459274-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7186512-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2008521413-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7901880-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7910296-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8088581-B2 |
| priorityDate |
2002-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |