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filingDate 2003-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2003-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-03062791-A2
titleOfInvention Detection of single nucleotide polymorphisms using planar waveguides
abstract The present invention relates to a method of detecting single nucleotide polymorphisms in a gene of interest. A plurality of probes is immobilized on a planar waveguide. The probes comprise sequences complementary to a wildtype sequence of the gene of interest and complementary to a sequence of a known SNP in the gene of interest. A fluorescently-labeled analyte is flowed over the planar waveguide. The binding between the labeled analyte and each of the probes causes a change in the fluorescence signal. The SNP is detected by comparing the hybridization kinetics of the analyte with each of the probes. A method of detecting single nucleotide polymorphisms in a gene of interest by sequencing by hybridization is also disclosed.
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