| abstract |
A method of ligating nucleic acids characterized in that a single-stranded RNA and a single-stranded DNA, which have sequences complementary to each other, are annealed and then treated with an RNA ligase; a nucleic acid construct which is bonded to a target mRNA and then translated so as to give a ligation product of the target mRNA to a protein encoded thereby; a method of screening a nucleic acid and/or a protein using a ligation product of a target mRNA and a protein encoded thereby; a puromycin derivative subjected to the final deblocking by an enzyme reaction; a carrier protein useful in functionally expressing a target peptide or a target protein having relatively short amino acid residues (for example, a peptide library made up of random sequences) in a cell-free translation system; and a method of ligating two single-stranded or double-stranded DNAs, which have sequences complementary to each other and are in different types, in the absence of any primers. Thus, it becomes possible to synthesize an in vitro virus genome, which can be produced at only a poor efficiency by the conventional methods, within a short period of time at a high efficiency. |