| abstract |
The present invention relates to a process for producing a secretory protein using yeast, to a vector used therefor, and to yeast comprising the vector. Specifically, the present invention relates to a process for producing various secretory proteins including naturally-occurring human granulocyte-colony stimulating factor (hereinafter referred to as G-CSF) by providing a yeast expression vector possessing a novel complex secretory signal which facilitates secretion and extracellular expression, and especially by providing a novel gene sequence of human G-CSF protein by the use of yeast preference codons which increase the translation efficiency of yeast. |