Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c6425d817a68173767787c13936af7b5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e20d9c76a5da339e163c942b2b2a1a4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4ffc4e7c45ddd75fc62adab9bdba1e7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_11b5b679df97eb51c9a219509b45af28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_196fb1a63124a106d8d522fd92e0a996 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_259045c6eba6e2c5d338b84e0a88b571 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8213 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8214 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-82 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H1-00 |
filingDate |
2002-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14df86e6dc405e1f67ffcdde856276cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b27ac392c6e16060a0ff584fd532be1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69f1bf7a131322ebc60ab2ab5bf240ec http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5246b885be27a130a3673bedd3e5f214 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41775ab0c78688bd1e9ed6f8fc856166 |
publicationDate |
2003-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-03060137-A1 |
titleOfInvention |
Method for recombinating plastid using procaryotic recombinase gene |
abstract |
The objective of this invention is to enhance the efficiency of plastid transformation using nuclear transformed plants in which the microbial recombinase A(recA) is to target to (or expressed in) the plastid. This invention will be better explained by the following detailed descriptions. A plant is transformed with a nuclear transformation vector containing the microbial recA gene added with a plastid targeting sequence. In this nuclear transformed plant, the frequency of plastid transformation is enhanced greater than two-folds due to increased homologous recombination between the plastid transformation vector carrying genes of interest (or target genes) and the plastid genome. In addition, because plastid transformation is accomplished through a gradual process, adventitious shoots selected after being subjected to plastid transformation should be cut into explants, and then shoots regenerated from the explants are to be reselected until all of the plastids in the shoots are uniformly transformed. However, when the nuclear transformed plant is used, the number of reselection is reduced to 1/2 to 1/3 due to increased homologous recombination. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7235711-B2 |
priorityDate |
2002-01-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |