http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03048191-A3
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e6feb048bb6aa558dfb54db6876f2eba |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-26 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 |
| filingDate | 2002-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9d1db219320fbc7bae311287b4fd810 |
| publicationDate | 2003-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-03048191-A3 |
| titleOfInvention | Process for the selective alkylation of -sh groups in proteins and peptides for the study of complex protein mixtures |
| abstract | Weakly basic molecules containing a double bond (in particular vinylpyridines) are able to react and selectively alkylate -SH groups in proteins, thus preventing their re-oxidation to disulphur bridges. Contrary to conventional alkylating agents, such as iodoacetamide, such molecules reach 100% alkylation of all -SH residues, even in complex proteins, without reacting with other functional groups. Their use is particularly effective in proteome analysis and more generally for analysing proteins in which the -SH groups should be blocked. Additionally, the use of vinylpyridines partially or totally deuterated, and thus with a mass difference as compared to non-deuterated vinylpyridines, allows studies of induction/repression of protein synthesis. |
| priorityDate | 2001-12-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.