http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03026712-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1010e3862914acdd087e73d76911ec6d http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3183ca77093d4ac9e1e54305fd2a72dc |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0697 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61L27-3895 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61L27-20 |
| filingDate | 2002-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09e9c99dd89bb025808c95c1a56fa503 |
| publicationDate | 2003-04-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-03026712-A1 |
| titleOfInvention | Method of preparing basement membrane, method of constructing basement membrane specimen, reconstituted artificial tissue using the basement membrane specimen and process for producing the same |
| abstract | A basement membrane is formed by culturing cells on a substrate wherein the basal face of cells capable of forming a basement membrane has been coated with a polymer having a sugar chain capable of localizing a receptor having an effect of accumulating basement membrane-constituting components. The basement membrane specimen is constructed by treating cells, which are capable of forming a basement membrane and have been adhered to a support via the basement membrane, with a surfactant to solubilize lipid components of the cells and solubilizing proteins remaining on the basement membrane surface with the use of a mixture of an alkali solution with a protease inhibitor. An artificial tissue is obtained by inoculating and culturing desired cells capable of forming a basement membrane. Using a hydrophobic bond adsorption polymer having a linear carbon skeleton with a hydrophobic nature and a functional group capable of reacting with a protein (for example, an alternate copolymer of methyl vinyl ether with maleic anhydride), a protein support is tentatively adhered to a plastic surface and a basement membrane specimen or an artificial tissue is formed thereon. Thus, the protein support carrying the basement membrane specimen or the artificial tissue thereon can be physically separated from the plastic surface when needed. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008105454-A1 |
| priorityDate | 2001-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.