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filingDate 2002-09-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2003-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-03023571-A2
titleOfInvention Methods for differential cell counts including related apparatus and software for performing same
abstract The present invention provides an optical method, system and software for imaging cells, in particular blood cells. In one embodiment, laboratory samples containing blood cells are deposited onto bio-discs, which are specially manufactured discs with mixing chambers that contain specific antigens to lock down various components of the blood cells. Once in the optical drive, the disc is spun and the samples and antigens are mixed with other solutions. Electromagnetic beams are then directed at the bio-disc to interact with the samples at specific capture zones and the resulting beams are collected by a detector. The information contained in the beams is then sent to a processor that produces a digital image. Various image processing methods such as binarization, background uniformization, normalization and filtering are performed to enhance cells in the investigational data for accurate counting. Other techniques are designed to correct for irregularities such as bubbles and dim cells.
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