| abstract |
The present invention provides a method for rapid identification of sites of insertion of DNA in to a cellular chromosome. The present invention also provides a gene trap vector. In one embodiment, the method of the present invention comprises the steps of stably transfecting a population of cells with a gene-trap vector, identifying cells with a trapped gene, distributing sorted cells into a matrix format, pooling cells from the matrix into discrete pools, producing cDNA sequence tags from the trapped genes in the pooled cells, making concatamers for each pool, cloning and sequencing the concatamers, defining the sequence tag of each well in the matrix. |