| abstract |
The invention relates to devices, devices for arraying biomolecules, including cells, methods for arraying biomolecules, assays for monitoring cellular movement, and systems for monitoring cellular movement. The devices include a support (140), a first layer (150) configured to be placed in fluid-tight contact with the support, the first layer (150) having an upper surface (140a) and defining a pattern of micro-orifices (300), each micro-orifice of the pattern of micro-orifices (300) having walls (150a) and defining a micro-region on the support (140) when the first layer (150) is placed in fluid-tight contact with the support such that the walls (150a) of each micro-orifice (300) and the micro-region on the support (140) together define a micro-well (141); and a second layer (160) configured to be placed in fluid-tight contact with the upper surface of the first layer (150), the second layer (160) defining a pattern of macro-orifices (170), each macro-orifice (170) of the pattern of macro-orifices (170) having walls and defining a macro-region when the first layer is placed in fluid-tight contact with the support (140) and the second layer (160) is placed in fluid-tight contact with the first layer (150) such that the walls of the macro-orifice and the macro-region together define a macro-well (151). |