| abstract |
A method of producing a fusion polypeptide in, and purifying the fusion polypeptide from, bacteria, the method comprises introducing into the bacteria an expression construct encoding the fusion polypeptide which comprises a TAT-derived peptide, and a protein-of-interest, whereby the TAT-derived peptide serves for transport of the fusion polypeptide from the bacterial cytoplasm to the periplasm. The fusion polypeptide is then substantially exclusively purified from the bacterial periplasm. |