| abstract |
The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. The invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e.g., transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated form nucleic acids isolated from more than one organism for enzyme, e.g., transaminase, activities are also provided. |