| abstract |
Glicosaminoglycans derived from K5 polysaccharide having high antithrombin activity and useful for the control of coagulation and as antithrombotic agents are obtained starting from an optionally purified K5 polysaccharide by a process comprising the steps of N-deacetylation/N-sulphation, C5 epimerisation of at least 40% of the glucuronic acid moiety, O-oversulphation, selective O-desuphation, 6-O-sulphation, N-sulphation, and optional depolymeration, and in which the selective O-desulphation step is carried out by treatment of the oversulphated product with a mixture dimethyl sulfoxide/methanol at a temperature of 50-70oC for 135-165 minutes. New, particularly interesting antithrombin compounds are obtained by thus controlling the reaction time in the selective O-desulphation step and submitting the product obtained at the end of the final N-sulphation step to depolymeration. |