Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fdce00614a7e3df9150f10db63ad7532 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d68b23075355b837c49ee62f10863db7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ab13d8b9e253e23f562b7494f8614068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_20a506406619e64ae6e0b27b47af2a2b http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_287114022fd18ac32942a7d58b0ea658 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1ba70424730c7e832c45aa93b862804 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24222 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-00 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-506 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 |
filingDate |
2001-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f64d6577e407dc62156b8f81ed3e766 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f66a579cfd14c132d4213c2233e810b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bcb08b8ebfa691eabe14aeea57ff2764 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d91d98b90f35daa50c79aca36852faf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4c4cad3ad792850730845860fec5c54 |
publicationDate |
2002-06-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-0248375-A2 |
titleOfInvention |
Purified active hcv ns2/3 protease |
abstract |
A method for producing a refolded, inactive form of recombinantly produced NS2/3 protease which comprises the steps of: a) purifying the protease from inclusion bodies in the presence of a chaotropic agent; and b) refolding the purified protease by contacting it with a reducing agent and lauryldiethylamine oxide (LDAO) in the presence of reduced concentration of chaotropic agent or polar additive. The invention further comprises a method for activating this refolded inactive NS2/3 protease by adding an activation detergent. This method produces large amounts of the active NS2/3 protease to allow small molecules and ligands to be screened as potential inhibitors of NS2/3 protease, which may be useful as therapeutic agents against HCV. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7655394-B2 |
priorityDate |
2000-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |